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GANT 58

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Purity:99.88%

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COA HPLC HNMR

GANT 58

Catalog No. T15370Cas No. 64048-12-0

GANT 58 (NSC-75503) is a potent antagonist of Gli. Which inhibits GLI1-induced transcription (IC50: 5 μM).

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Pack Size	Price	Availability
5 mg	$40	In Stock
10 mg	$64	In Stock
25 mg	$119	In Stock
50 mg	$197	In Stock
100 mg	$318	In Stock
1 mL x 10 mM (in DMSO)	$44	In Stock

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Product Introduction

Bioactivity

Description	GANT 58 (NSC-75503) is a potent antagonist of Gli. Which inhibits GLI1-induced transcription (IC50: 5 μM).
In vitro	GANT58 is a downstream inhibitor of Hh signaling and it also is an indeed inhibitor of Hh signaling downstream of Smo and Sufu. GANT58 potently inhibits in vitro tumor cell proliferation in a GLI-dependent manner and successfully blocks cell growth using human prostate cancer cells harboring downstream activation of the Hh pathway[1]. GANT58 has been shown to inhibit transcriptional activation by GLI1 (as well as by the other GLI species) and it has been shown to inhibit GLI transactivation[2]. GANT58 mainly acts at the nuclear level because transcription induced by GLI1 with a mutated nuclear export signal is still blocked.
In vivo	Nude mice are treated with every second-day s.c. injections at a concentration of 50 mg/kg of cyclopamine, GANT61, GANT58, or solvent only (n=4-5 for each group). Although mice treated with these compounds showed no such signs of toxicity, this protocol is also introduced for the GANTs to be able to compare all compounds. All injections are done 2-3 cm away from the tumors. Suppression of tumor cell growth is observed for all compounds, during an 18-day treatment period. Treatment with cyclopamine or GANT58 results in the inhibition of additional xenograft growth and limited the increase in tumor size[1].
Alias	NSC 75503

Chemical Properties

Molecular Weight	392.48
Formula	C24H16N4S
Cas No.	64048-12-0

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 9.09 mg/mL (23.16 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5479 mL	12.7395 mL	25.4790 mL	127.3950 mL
5 mM	0.5096 mL	2.5479 mL	5.0958 mL	25.4790 mL
10 mM	0.2548 mL	1.2740 mL	2.5479 mL	12.7395 mL
20 mM	0.1274 mL	0.6370 mL	1.2740 mL	6.3698 mL

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Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

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mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

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